MAGUS Lum VD500L Fluorescence Inverted Digital Microscope
With a camera. Magnification: 100–400x. Trinocular head, plan achromatic objectives, 5W LED transmitted light and reflected light illumination, Köhler illumination
Product ID | 83022 |
Brand | MAGUS |
Warranty | 5 years |
EAN | 5905555018249 |
Package size (LxWxH) | 15x24x32.3 in |
Shipping Weight | 58.9 lb |
The MAGUS Lum V500L is an inverted microscope for fluorescence microscopy in the reflected light and brightfield and phase contrast microscopy in the transmitted light. DAPI, FITC, and TRITC fluorochrome filters are used for fluorescence microscopy. The inverted design allows you to study samples in laboratory ware such as petri dishes, flasks, etc. The height of the lab ware can be 55mm or 165mm when the stand is tilted. The optics are designed for lab ware with a bottom thickness of 1.2mm. The microscope is ideal for research and routine laboratory work and it can be used for educational purposes.
Digital Camera
The MAGUS CLM90 digital camera is designed for use in fluorescence and darkfield microscopy techniques.
The camera is equipped with a 7.1MP sensor and produces realistic images at a resolution of 3200x2200px. The camera is recommended for use with 4x, 10x, 20x, and 40x objectives. When working with low magnification objectives, the camera will allow you to see the finer details.
Video is recorded at 51.3fps or 133.8fps depending on its resolution. Videos are smooth with soft and subtle transitions between frames. The movement of the sample is displayed in real time with no delays. The camera makes it easy to work with objects that are moving and is suitable for classroom demonstrations.
The camera is equipped with a USB3.0 interface. The data transfer speed is 10 times faster than USB2.0 cameras. The high-speed camera is recommended for professional laboratories, research, or university training.
Optics
Two options for mounting a digital camera and monitor: The trinocular tube is for mounting a monitor (not included), and the side port on the body is for mounting a digital camera (not included). There is beam splitting: 100/0 or 0/100 (on the body) and 100/0 or 50/50 (trinocular head). The 45° eyepiece tube inclination angle allows for observation with maximum comfort. When assembling the microscope, the user can rotate the eyepiece tubes 180°.
The set includes six plan achromatic objectives: three of them for brightfield and fluorescence microscopy, and another three for phase-contrast microscopy. They are infinity-corrected and have a long working distance. The revolving nosepiece has six slots for mounting objectives. The revolving nosepiece is located under the stage on a stand.
Illumination
The fluorescence excitation source is a 5W LED with 50,000 hours of lifetime without replacement. There are three excitation filters: DAPI, FITC, and TRITC fluorochrome filters. LED illumination is more energy efficient and easier to maintain than mercury illumination, which is why it is recommended for use in universities. LED illumination provides color consistency. The LEDs turn on and off quickly and do not overheat during long hours of operation.
The source of the transmitted light is also a 5W LED. It is bright enough for brightfield and phase contrast microscopy techniques with each objective.
Both reflected and transmitted illumination can be adjusted according to the Köhler method. It allows you to reach the maximum resolution limit for each objective lens and get a clear, sharp image without artifacts or darkening at the edges.
The microscope is equipped with a phase-contrast condenser: Three slots are used for phase objectives with magnifications of 10x, 20x, and 40x, and the fourth slot is for brightfield microscopy. Phase rings can be centered. You can switch between the microscopy techniques by rotating a dial.
Stage and focusing mechanism
The fixed stage is equipped with a mechanical attachment for moving laboratory ware and three cup holders of different sizes. The lab ware can be moved in two mutually perpendicular directions in the horizontal plane. The microscope has a coarse and fine focusing mechanism. The coarse focusing mechanism has a lock knob and tension adjustment. The knobs are ergonomically designed to allow the user to maintain a relaxed posture while working.
Accessories
The microscope can be equipped with additional eyepieces, calibration slides, or a digital camera from the MAGUS line of accessories.
Key features of the microscope:
- Inverted design for observing samples in lab ware with a bottom thickness of 1.2mm and samples of up to 55mm height in as well as up to 165mm height if the stand is tilted up
- Microscopy techniques: fluorescence (reflected light), brightfield and phase-contrast (transmitted light)
- Köhler illumination setup for transmitted and reflected light; long-lasting 5W LEDs without replacement as light sources
- There are three excitation filters: DAPI, FITC, and TRITC fluorochrome filters
- Phase-contrast condenser for quick and convenient switching between the microscopy techniques
- Trinocular head; two options to mount a camera or monitor: the 100/0 or 0/100 beam splitting (on the body), 100/0 or 50/50 beam splitting (on the head)
Key features of the camera:
- For fluorescence and darkfield microscopy with 4x, 10x, 20x, or 40x objectives
- 7.1MP resolution: When observing with low magnification objectives, the camera will enable you to see finer details
- 51.3fps and 133.8fps at 3200x2200px and 1584x1100px resolutions for observing moving objects, recording video and moving the preparation without jerkiness and delays
- Peltier element reduces the sensor temperature during prolonged operation and eliminates thermal noise and, therefore, long shutter speeds can be used when taking photos
- Global shutter for fast signal readout from the sensor, increased image brightness, no false optical effects when observing moving objects
- SONY Exmor monochrome CMOS backlit sensor provides low noise level and high light sensitivity even in low-light conditions. You will get clearer, brighter, and more color-saturated images
- USB3.0 interface for fast, smooth data transfer
- Software with photo, video recording, editing, external display functions, linear and angular measurements
The kit includes:
- MAGUS CLM90 Digital Camera (digital camera, USB cable, 12V, 3A adapter, case, Installation CD with drivers and software, user manual and warranty card)
- Base with a built-in power input, reflected light illuminator, transmitted light source, condenser, focusing mechanism, stage, and revolving nosepiece
- Trinocular head
- Infinity plan achromatic objective: PLL 10x/0.25 phase WD 4.3 mm
- Infinity plan achromatic objective: PLL 20х/0.40 WD 8.0mm
- Infinity plan achromatic objective: PLL 40х/0.60 WD: 3.5mm
- Infinity plan achromatic objective: PLL 10x/0.25 PHP2 WD 4.3mm
- Infinity plan achromatic objective: PLL 20x/0.40 PHP2 WD 8.0mm
- Infinity plan achromatic objective: PLL 40x/0,60 PHP2 WD 3.5mm
- Eyepiece 10x/22mm with long eye relief (2 pcs.)
- UV shield
- C-mount adapter 1x
- Hex key wrench
- Power cord
- Dust cover
- User manual and warranty card
Available on request:
- 10x/22mm eyepiece with a scale
- 12.5x/14mm eyepiece (2 pcs.)
- 15x/15mm eyepiece (2 pcs.)
- 20x/12mm eyepiece (2 pcs.)
- 25x/9mm eyepiece (2 pcs.)
- Calibration slide
Product ID | 83022 |
Brand | MAGUS |
Warranty | 5 years |
EAN | 5905555018249 |
Package size (LxWxH) | 15x24x32.3 in |
Shipping Weight | 58.9 lb |
Type | biological, light/optical |
Head | trinocular |
Nozzle | Siedentopf, rotatable 180° |
Magnification, x | 100 — 400 |
Magnification, x (optional) | 40–500/600/800/1000 |
Eyepiece tube diameter, in | 1.2 |
Eyepieces | 10х/22mm, eye relief: 10mm (*optional: 10x/22mm with scale, 12.5x/14; 15x/15; 20x/12; 25x/9) |
Objectives | infinity plan achromatic: PLL 10x/0.25/4.3; PLL 20x/0.40/8.0; PLL 40x/0.60/3.5; phase: PLL 10x/0,25/4,3 PHP2; PLL 20x/0.40/8.0 PHP2; PLL 40x/0.60/3.5 PHP2; parfocal distance: 45mm |
Revolving nosepiece | for 6 objectives |
Working distance, mm | 4.3 (10х); 8.0 (20x); 3.5 (40x) |
Interpupillary distance, in | 1.9 — 3 |
Stage, mm | 227x208 |
Stage moving range, mm | 77/134.5 |
Stage features | fixed, with glass plate Ø118mm and mechanical attachment; dish holders: 86x129.5, Ø90mm; 34x77.5mm, Ø68.5mm; 57x82mm, Ø60mm; 29x77.5mm, Ø35mm |
Condenser | NA 0.6, working distance: 55mm; phase contrast turret; with locking screws |
Diaphragm | aperture iris diaphragm |
Focus | coaxial coarse and fine focusing knobs located on both sides |
Illumination | fluorescent, halogen |
Brightness adjustment | ✓ |
Power supply | AC network, 85–265V, 50/60Hz (US type plug adapter is not included) |
Light source type | reflected light: 5W LED; transmitted light: 5W LED |
Light filters | yes |
Operating temperature range, °F | 41...+95 |
Special features | phase contrast condenser (turret) with a free slot and phase annuli plates for 10x, 20x and 40x objectives; centering auxiliary microscope |
User level | experienced users, professionals |
Assembly and installation difficulty level | complicated |
Fluorescent module | filters: ultraviolet (UV), violet (V), blue (B), green (G) |
Fluorescence filter: filter type, excitation wavelength/dichroic mirror/emission wavelength | ultraviolet (UV), 320–380nm/425 nm/435 nm; violet (V), 380–415nm/455nm/475nm; blue (B), 450–490nm/505nm/515nm; green (G), 495–555nm/585nm/595nm |
Application | laboratory/medical |
Illumination location | dual |
Research method | bright field, fluorescence, phase-contrast microscopy |
Pouch/case/bag in set | dust cover |
Camera specifications | |
Sensor | SONY Exmor CMOS |
Color/monochrome | monochrome |
Megapixels | 7.1 |
Maximum resolution, pix | 3200x2200 |
Sensor size | 1.1" (14.4x9.9mm) |
Pixel size, μm | 4.5x4.5 |
Two-stage thermoelectric module (Peltier element) to set the temperature 42°C below room temperature | ✓ |
Light sensitivity | 3354mV with 1/30s |
Signal/noise ratio | 0.15mV at 1/30s |
Exposure time | 0.1ms–1h |
Video recording | ✓ |
Frame rate, fps at resolution | 51.3@3200x2200, 133.8@1584x1100 |
ADC digit capacity (bit) | 8/12 (selectable) |
Image format | *.jpg, *.bmp, *.png, *.tif |
Video format | output: *.wmv, *.avi, *.h264 (Windows 8 and later), *h265 (Windows 10 and later) |
Shutter type | Global shutter |
White balance | manual, automatic |
Exposure control | manual, automatic |
Software features | image size, brightness, exposure time |
Software | MAGUS View |
Output | USB 3.0, 5Gb/s |
System requirements | Windows 8/10/11 (32bit and 64bit), Mac OS X, Linux, up to 2.8GHz Intel Core 2 or higher, minimum 2GB RAM, USB 3.0 port, CD-ROM, 17" or larger display |
Mount type | C-mount |
Body | CNC aluminum alloy |
Camera power supply | DC, 5V, from computer USB port; a 12V, 3A adapter for Peltier element |
Camera operating temperature range, °F | -10...+50 |
Operating humidity range, % | 30 — 80 |
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